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For analysis of neutrophil ROS after co-culture, LNCaP, C42B, PC3, or RM1 cells were plated in triplicate in a 24 well plate

They contribute to collagen production, matrix organisation and tendon structure

7a, b, Cys 2 starvation as well as BSO and erastin treatment increased the percentage of cells in the G0/G1 phase with a concomitant decrease in the G2 phases, suggesting that GSH depletion induces growth arrest in RPE cells

7b), confirming our hypothesis

Recent work reveals that GPX4-controlled ferroptosis in neutrophils drives systemic autoimmunity by unleashing redox-active iron, which amplifies inflammatory and immune activation, a hallmark of SLE (216)